Speaker : Dr. Denina Simmons – University of Ontario Institute of Technology
Title : Environmental Proteomics And Toxicology
Proteomic methods can be used to assess the effects of contamination on biota, to determine protein biomarkers that are specific to environmental exposures, and also to characterize unique mechanisms of action by contaminants. Untargeted plasma proteomic analysis offers many unique advantages: it provides a snapshot of whole organism health, sample preparation is easy, and there is potential for non-lethal sampling methods. A highly cost effective and efficient method for analyzing the proteome using formic acid protein digestion and liquid chromatography mass spectrometry was developed and applied to understand the effects of various organic contaminants on teleost fish and aquatic insects. This research seminar will introduce the novel and paradigm-shifting untargeted proteomics approach, describe the technical methods, and present various laboratory and field studies that demonstrate the great potential and applicability of large proteomic datasets within the field of organic toxicology. Specifically, this seminar will discuss results from rainbow trout exposed to the organic flame retardant TBBPA; the mechanism of action of the anti-inflammatory drug diclofenac in the South American catfish; and finally the remarkable effects of pharmaceutical mixtures present in waste water effluents on the plasma proteome, plasma metabolome, and behaviour of goldfish.